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One of the primary sources of acetyl-CoA is from the breakdown of sugars by glycolysis which yield pyruvate that in turn is decarboxylated by the pyruvate dehydrogenase complex generating acetyl-CoA according to the following reaction scheme:ĬH 3C(=O)C(=O)O − pyruvate + HSCoA + NAD + → CH 3C(=O)SCoA acetyl-CoA + NADH + CO 2 The NADH and FADH 2 generated by the citric acid cycle are, in turn, used by the oxidative phosphorylation pathway to generate energy-rich ATP. The reactions of the cycle also convert three equivalents of nicotinamide adenine dinucleotide (NAD +) into three equivalents of reduced NAD + (NADH), one equivalent of flavin adenine dinucleotide (FAD) into one equivalent of FADH 2, and one equivalent each of guanosine diphosphate (GDP) and inorganic phosphate (P i) into one equivalent of guanosine triphosphate (GTP). Through catabolism of sugars, fats, and proteins, the two-carbon organic product acetyl-CoA is produced which enters the citric acid cycle. The reactions of the cycle are carried out by eight enzymes that completely oxidize acetate (a two carbon molecule), in the form of acetyl-CoA, into two molecules each of carbon dioxide and water. The citric acid cycle is a key metabolic pathway that connects carbohydrate, fat, and protein metabolism. Structural diagram of acetyl-CoA: The portion in blue, on the left, is the acetyl group the portion in black is coenzyme A. The citric acid cycle itself was finally identified in 1937 by Hans Adolf Krebs and William Arthur Johnson while at the University of Sheffield, for which the former received the Nobel Prize for Physiology or Medicine in 1953, and for whom the cycle is sometimes named the "Krebs cycle". Because this tissue maintains its oxidative capacity well after breaking down in the Latapie mill and releasing in aqueous solutions, breast muscle of the pigeon was very well qualified for the study of oxidative reactions. He made this discovery by studying pigeon breast muscle. Several of the components and reactions of the citric acid cycle were established in the 1930s by the research of Albert Szent-Györgyi, who received the Nobel Prize in Physiology or Medicine in 1937 specifically for his discoveries pertaining to fumaric acid, a key component of the cycle.
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